A snapshot and pipeline for tissue-specific gene expression meta-analysis in honey bees

Catalog Row47

Citation KeyFriedman2023SnapshotPipelineTissueSpecific047

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Overview

Extracted from the local paper documentation when available.

This study investigates the role of coding sequence changes in the evolution of postdevelopmental novelty in honey bees, challenging the traditional evo-devo paradigm. Through RNA-Seq experiments, we demonstrate that positively selected tissue-specific genes, particularly taxonomically restricted genes (TRGs), are crucial for novel physiological functions in adult honey bees.

honey beesApis melliferagene expressionRNA-Seqnovel traitstaxonomically restricted genescoding sequence evolution

Use Notes

Concise findings and methods pulled from README/SKILL documentation.

Findings / Concepts

Identified the significance of coding sequence changes in the evolution of adult phenotypic novelty in honey bees.
Demonstrated that positively selected tissue-specific genes underlie novel physiological functions.
Highlighted the role of taxonomically restricted genes (TRGs) in conferring specialized cellular functions.
Challenged the evo-devo paradigm by showing that low network connectedness in genes facilitates rapid coding sequence changes.

Methods / Techniques

Conducted ten RNA-Seq experiments across various tissues in honey bees.
Analyzed gene expression patterns to identify highly expressed taxonomically restricted genes (HE-TRGs).
Examined differential gene expression between nurses and foragers to identify genes involved in social functions.
Utilized statistical tests to assess the significance of TRG expression in novel versus conserved tissues.

Citation

Plain-text citation for quick reuse.

Friedman, Daniel Ari. 2023. A snapshot and pipeline for tissue-specific gene expression meta-analysis in honey bees. Zenodo.

Primary source Documentation BibTeX