# 📄 Influence of Nuclear Structure on formation of radiation-induced lethal lesions

**Daniel A. Friedman, Daniel F. Pilch** (2016) · *Int. J. Radiation Biology*

[![DOI](https://img.shields.io/badge/DOI-10.3109%2F09553002.2016.1144941-blue)](https://doi.org/10.3109/09553002.2016.1144941)

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{"@context":"https://schema.org","@type":"ScholarlyArticle","headline":"NuclearStructure","abstract":"Ionizing radiation causes DNA double-strand breaks and disrupts chromatin architecture, potentially leading to chromosomal aberrations and genomic instability. Chromosome conformation capture (3C) technologies, particularly Hi-C, have revolutionized our understanding of three-dimensional genome organization. Here we review the intersection of radiation biology with chromatin conformation studies, examining how radiation impacts nuclear organization at multiple scales from nucleosomes to chromoso","keywords":["Hi-C","chromosome conformation","radiation biology","chromatin architecture","DNA damage","topologically associating domains","nuclear organization","3C technologies"],"author":{"@type":"Person","name":"Daniel Ari Friedman","url":"https://danielarifriedman.com/"}}
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## Abstract

> This paper discusses the influence of nuclear structure on the formation of radiation-induced lethal lesions, particularly focusing on the rejoining of fragmented nuclear DNA caused by ionizing radiation. It synthesizes existing knowledge with new insights from high-throughput sequencing data to better understand how spatial organization within the genome affects the generation of lethal chromosome aberrations.

## Keywords

`Hi-C` · `chromosome conformation` · `radiation biology` · `chromatin architecture` · `DNA damage` · `topologically associating domains` · `nuclear organization` · `3C technologies`

## Key Contributions

- Synthesis of existing knowledge with new insights from high-throughput sequencing data.
- Identification of the fractal globule organization of DNA and its implications for radiation damage.
- Exploration of the spatial proximity of transcriptionally active euchromatin and its susceptibility to radiation.
- Discussion of the temporal factors influencing the formation of lethal lesions post-irradiation.

## Methods

- High-throughput sequencing of chromosome conformation capture experiments.
- Analysis of spatial organization of DNA within the nucleus.
- Examination of transcriptional programs linking genomic locations.
- Review of existing models of radiation-induced DNA damage and repair mechanisms.

## 🎯 Consulting & Tutoring

**Available for AI Research Consulting and Tutoring.** [Contact Daniel Ari Friedman, PhD](https://danielarifriedman.com/) for collaboration on Active Inference, Bayesian modeling, and computational biology.

## Citation

```bibtex
@article{2016_NuclearStructure,
  author = {Daniel A. Friedman, Daniel F. Pilch},
  title = {{Influence of Nuclear Structure on formation of radiation-induced lethal lesions}},
  journal = {Int. J. Radiation Biology},
  year = {2016},
  doi = {10.3109/09553002.2016.1144941},
}
```

## File Inventory

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- `README.md` (2,188 bytes)
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